Clinical ProceduresQuestion 47 of 100
Which method most reliably confirms that an autoclave is actually killing microorganisms?
a.Autoclave indicator tape that changes color on the outside of the pack
b.A biological indicator, or spore test, run at the intervals required by policy
c.Checking that the door seal looks clean
d.Observing steam escaping from the chamber during the cycle
Explanation
A biological indicator containing resistant bacterial spores is the only method that proves sterilization conditions were sufficient to kill microorganisms, and it is run on a scheduled basis. Indicator tape only shows the pack was exposed to heat, not that sterilization was achieved. A clean door seal and visible steam are maintenance and operating observations, not proof of sterility.
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