Collection ProceduresQuestion 35 of 100

An order requires a lavender EDTA tube and a gray fluoride tube only. Which sequence is correct and why?

a.Lavender first, because EDTA precedes the glycolytic inhibitor tube in the order of draw
b.Gray first, because it is the smallest tube
c.Either order, because neither additive affects the other
d.Gray first, because the fluoride preserves glucose better when drawn early

Explanation

In the standard sequence the EDTA tube always precedes the sodium fluoride and potassium oxalate tube, which is drawn last. Potassium oxalate carried backward into an EDTA tube can distort cell morphology and hematology results. Tube size and how early the fluoride is drawn have no bearing on the required order.

Law Reference: CLSI GP41

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