Specimen HandlingQuestion 61 of 100

A neonatal bilirubin is collected. How must the specimen be handled during transport?

a.Frozen immediately at minus 20 degrees Celsius
b.Left uncovered at room temperature to allow clotting
c.Kept in an ice slurry for at least 30 minutes
d.Protected from light, for example with an amber tube or foil wrap

Explanation

Bilirubin is photosensitive and degrades within minutes of light exposure, so an amber microtube or foil wrapping is used. Vitamin B12, folate, carotene and vitamin A require the same protection. Freezing whole blood hemolyzes it, chilling is not the required protection here, and leaving the tube exposed causes falsely low results.

Law Reference: CLSI GP44

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