Specimen HandlingQuestion 64 of 100

Which collection practice is most likely to hemolyze a specimen?

a.Forcing blood from a syringe through the needle into an evacuated tube
b.Inverting an EDTA tube gently eight times
c.Using a 21 gauge needle on a good antecubital vein
d.Letting the alcohol dry fully before puncture

Explanation

Pushing blood through a narrow needle under pressure shears red cells and is a classic cause of hemolysis, which is why a transfer device is used instead. Gentle inversion, an appropriately sized needle and fully dried alcohol all reduce hemolysis. Hemolysis falsely raises potassium, LDH, AST and magnesium and often forces a redraw.

Law Reference: CLSI GP41

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